Biocrates Life Sciences AG

on . Posted in SME

BiocratesTargeted metabolomics analysis of bio-fluids using a combined flow injection & LC-MS/MS assay

All characteristic metabolic properties of a cell, tissue or an organism are described by its metabolome, which is the functional readout of the genome and proteome. Metabolomics is the next member in the "omics" cascade investigating the metabolome within a specific biological matrix (e.g. serum, tissue, cells or CSF). Combining the knowledge of related "omics" disciplines, like genomics, transcriptomics, and proteomics, metabolomics opens the unique chance being closer to the phenotype than ever before and will lead for the first time to a comprehensive understanding of biochemical pathways in life systems.
For quantifying a wide range of metabolites in complex biological samples, LC-ESI-MS/MS is becoming one method of choice. However, the identification and quantification in particular of metabolites with high relevance in medicine and biochemistry is the current bottle-neck in metabolomics. Due to the diversity of metabolites, analytical demands raise extreme and time consuming difficulties in contrast to genomic or proteomic analyses. Targeted metabolomics will overcome these challenges for researchers by providing them with robust and easy to use assays for analytes or pathways of interest.

According to widely differing requirements in the field of metabolomic research, flow injection analysis (FIA-ESI-MS/MS) and LC-ESI-MS/MS based assays for multi parameter analyses were developed which comprise varying sets of analytes. The need of minimal sample volumes and the flexibility to varying biological matrices as well as analyte quantification over a wide spread range of concentrations is a high advantage of these ESI-MS/MS methods. By the use of our methods, over 600 analytes can be quantified easily and quality controlled with high reproducibility and accuracy. This set of analytes consists of the following classes: acylcarnitines, amino acids, biogenic amines, sugars, eicosanoids, lipids, steroids, neurotransmitters, bile acids, energy metabolism, oxysterols and vitamins.

Multi parameter analyses produce an extensive set of data which will even increase while quantifying numerous analyte classes simultaneously. The creation of specific software and database for handling and interpreting metabolomics results as well as combining them with other "omics" data is consequently necessary. Automated calculation of concentrations as well as easy and interactive data evaluation is done by MetIDQ™ software. In addition MetIDQ™ provides the possibility for fast detection of alterations in metabolite concentrations by graphic visualization. An integrated laboratory information management system (LIMS) is a user-friendly tool for data storage and organization.
Targeted metabolomics are commercially available fast and easy to use solutions for in depth investigations in many fields of basic and applied research, clinical biochemistry and personalized medicine. Newfound results from the field of metabolomics have the potential to revolutionize our understanding of complex human disease, clinical diagnostics, molecular mechanisms and biological pathways.